Properties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122
نویسندگان
چکیده
منابع مشابه
Properties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122.
Alcaligenes faecalis AE122 that used poly(3-hydroxybutyrate) (PHB) as a sole source of carbon was newly isolated from a coastal seawater sample. The strain required seawater for growth on PHB as well as in a nutrient broth, in which seawater could be replaced by an appropriate concentration of NaCl. PHB depolymerase was purified to homogeneity from the culture supernatant of A. faecalis AE122 b...
متن کاملStructure and function of poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1.
Poly(3-hydroxybutyrate) (PHB) depolymerase from Alcaligenes faecalis T1 is composed of three domains: the catalytic (C) domain, the fibronectin type III-like (F) domain, and the substrate-binding (S) domain. We constructed domain deletion, inversion, chimera, and extra-F-domain mutants and examined their enzyme activity and PHB-binding ability. In addition, we performed substitution of 214Asp a...
متن کاملCloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.
The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the 29 antibody-positive clones, 1 (pDP14), containing about 4 kilobase pairs of A. faecalis T1 DNA, ...
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This study investigated the potential of aquatic bacteria for their ability as a biocatalyst to synthesized Fe2O3 nanoparticles using iron precursor, FeCl3. A total of 25 aquatic bacterial strains were isolated in trypticase soy agar plus 10 mM FeCl3 with selective enrichment technique. Among the bacterial strains evaluated, NV06 was the only strain able to synthesize Fe2O3 nanoparticles extrac...
متن کاملEnzymatic degradation mechanism of a solution-grown poly[(R)-3-hydroxybutyrate] single crystal with an extracellular polyhydroxybutyrate depolymerase purified from Alcaligenes faecalis T1
We report the enzymatic degradation mechanism of a solution-grown poly[(R)-3-hydroxybutyrate] (P(3HB)) single crystal with an extracellular polyhydroxybutyrate depolymerase purified from Alcaligenes faecalis T1 (PhaZAfa). The P(3HB) single crystals adsorbed on highly ordered pyrolytic graphite were incubated with a wild-type or a hydrolytic-activity-disrupted mutant of PhaZAfa, and morphologica...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1995
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.61.5.1727-1730.1995